The University of New England has excellent poultry research and experimental facilities.
Animal house complex
Animal House Complex
- Eight environmentally controlled rooms (temperature and humidity controllable), each capable of accommodating 32 group cages for ME trials.
- One brooder room capable of holding 2000 birds up to 3 weeks of age.
- Twelve multi-purpose rooms – can be used for small type group trials or animal behaviour work.
- Four small (one layer or 1 to 6 broilers, depending on age) closed-circuit respiration calorimeter systems.
- 48 floor pens, 48 group cages, 96 individual cages, and 24 closed-circuit net energy chambers.
- Located on the north eastern side of the campus.
Semi-commercial cage system layer shed
- Three layer sheds with combined capacity of 8000 layers.
- Two shed with 200 single cages each.
- One large semi commercial shed with a capacity for 3000 layers.
- Brand new purpose-built free range facility with a total capacity for 3000 birds, 10 -20 replicates with movable partitions.
- 30 new renovated barn layer sheds with a capacity for 1800 birds.
Free range experimental facilities
- Located 15 km north of the campus.
- Three sheds, each capable of holding 3500 birds on the floor.
- Twenty-four isolator units in a climate controlled environment, with full HEPA filtration facilities for each unit, remote monitoring of each unit for temperature, humidity etc.
- PC2 certified from OGTR, experiments can be conducted with genetically modified organisms (e.g., vaccine).
- Ten small isolation units each holding up to 200 broilers on the floor or 24 layers in cages. Three of these sheds are positive pressure.
- Two large isolation sheds, each can hold 600 broilers or 300 layers in cages. A post-mortem unit is located on site.
External view of isolator facility
Internal view of isolator facility
Feed mixing facilities
- Feed mixing room attached to the Animal House complex.
- One hammer mill.
- Two cold pellet presses with capacities up to 180 kg/hr.
- A brand new feed mill with steam pelleting capacity
- Three feed mixers ranging in capacity from 2 kg to 600 kg.
- Balances and scales of various capacities.
- Proximate analyses (dry matter, ash, crude protein, crude fibre, NDF, ADF, lignin, total fat).
- Miscellaneous analyses.
- Nitrogen (Leco FP-2000 organic N analyser & classic Kjeldahl analyses)
- Mineral elements (ICP, UV/Vis spectroscopy)
- VFA (including lactate and succinate – GC)
- Gross energy (bomb calorimetry using IKA7000 systems)
- Various physiological & biochemical analyses (DADE automatic analyser and single and double-beam spectrophotometers)
- Electron microscopy (TEM, SEM, probe)
- NMR, GC-MS. LC-MS
- Carbohydrate analyses: glucose, total starch, digestible starch, resistant starch, total dietary fibre, total NSP, soluble NSP, insoluble NSP, free sugars, oligosaccharides, uronic acid, beta-glucan (mixed linkage).
- Specialised analyses: nutrient digestibility using marker and whole tract techniques, tannins, phytate, extract viscosity, digesta viscosity, enzyme activity.
Egg Quality Laboratory
- Contains a full set of equipment supplied by Technical Services and Supplies, U.K., in addition to some custom-made equipment.
- All egg quality measurements can be conducted: shell thickness, shell colour (reflectivity), shell breaking strength, egg specific gravity, albumen height, Haugh Units, yolk colour (colorimeter), shell deformation, shell weight, egg shape index, shell ultrastructure.
Gut Microbiology Laboratory
- Microbiological analyses of feed and digesta samples using non-selective and selective cultivation techniques.
- Isolation and characterisation of strict anaerobic bacteria.
- Evaluation of microbial fermentation products (gases and short-chained fatty acids including lactate and succinate).
- Molecular differentiation and identification of bacteria isolated from the gastrointestinal tract (PCR-based methods).
- In vitro quantification of microbial fermentation in different sections of the gastrointestinal tract.
- In vitro assessment of the survival of pathogenic bacteria in different sections of the gastrointestinal tract.
- In vitro assessment of adherence of bacteria to the gut wall in the small intestine(PIOC method).
- Full range of basic and advanced histological facilities and techniques including fluorescence with high resolution digital camera image capture.
- Digitization of structures using Video Pro and Spot softwares.
Molecular and Microarray Laboratory (PC2)
- Established at UNE under the Primary Industries Innovation Centre (PIIC).
- General molecular biology approaches:
- Automated DNA extractions
- RNA extractions
- PCR (high throughput)
- Robotic liquid handling
- Agarose/ polyacrylamide gel electrophoresis
- DNA cloning
- DNA sequencing
- Digital PCR bioassay
- Absolute quantification of DNA molecules
- Detection of rare alleles
- Quantification of rare organisms in environmental samples
- Gene expression:
- Quantitative realtime PCR
- Molecular marker system:
- Microsatellite analysis
- SNPs (High Resolution Melt curve analysis or HRM)
- Gene mapping:
- Linkage mapping (Joinmap etc)
- QTL mapping (QTL Express etc)
- Candidate gene identification and validation
- In situ hybridization:
- Western blot
- Major instruments:
- QuantStudio 3D digital PCR system
- 2100 Bioanalyzer
- QIAgility pipetting robot
- Nanodrop 8000
- Realtime PCR machine (Rotorgene 6000, Corbett)
- Microarray Scanner (Gene Pix Autoloader 4200 AL, Axon)
- CAS-1200 robotics (single channel precision liquid handling system, Corbett).
- X-tractor Gene robotics (automated nucleic acid extraction, Corbett).
- Other general molecular laboratory facilities.
Environmental Chemistry Analytical Facility (ECAF)
- Designed specifically for detecting pollutants (nutrients and metals) in soil, animal waste, and water.
- Large scale trial sites within close proximity to UNE campus.