Rapid tests for poultry diseases

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The following rapid tests for poultry diseases are available from the Asia Pacific Centre for Animal Health. These cost-effective tests are the result of a Poultry CRC project undertaken at The University of Melbourne.

» Download Submission Form for Poultry Samples Requiring Diagnostic PCR (PDF)

Disease	Causative agent	Test	Used for	Currently available
Infectious Coryza	Avibacterium paragallinarum	PCR	Detection	Yes
Coccidiosis	Eimeria species	PCR capilary electrophoresis	Detection and species ID	Yes
Coccidiosis	Eimeria species	Eimeria PCR HRM curve analysis	Detection and species ID	Yes
Coccidiosis	Eimeria species	Eimeria Taqman real time PCR	Detection and species ID	Yes
Infectious laryngotracheitis (ILT)	Herpesvirus	PCR	Detection	Yes
Infectious laryngotracheitis (ILT)	Herpesvirus	PCR-RFLP	Detection and strain ID	Yes
Infectious bronchitis virus (IBV)	Coronavirus	PCR	Detection	Yes
Infectious bronchitis virus (IBV)	Coronavirus	PCR-HRM curve analysis	Detection and strain ID	Yes
Colibacillosis	Escherichia coli	Multiplex PCR	Detection and virulent strain ID	Yes
Mycoplasmosis – all avian species	Mycoplasma synoviae	PCR	Detection	Yes
Mycoplasmosis – all avian species	Mycoplasma synoviae	PCR-HRM curve analysis	Detection and strain ID	Yes
Mycoplasmosis – all avian species	Mycoplasma gallisepticum	PCR	Detection	Yes
Mycoplasmosis – all avian species	Mycoplasma gallisepticum	PCR-HRM curve analysis	Detection and strain ID	Yes
Mycoplasmosis – turkeys	Mycoplasma meleagridis	PCR	Detection	Yes
Mycoplasmosis – ducks	Mycoplasma anatis	PCR	Detection	Yes
Fowl pox	Poxvirus	PCR	Detection	Yes
Chicken anaemia virus (CAV)	Circovirus	PCR	Detection	Yes
Inclusion body hepatitis (IBH)	Adenovirus	PCR-HRM curve analysis	Detection and strain ID	Yes
Chlamydiosis – all animal species	Chlamydia/Chlamydophila spp	PCR-HRM curve analysis	Detection and species ID	Yes
Avian Chlamydiosis	Chlamydophila psittaci	PCR-HRM curve analysis	Detection and strain ID	Yes
Marek’s disease (MD)	Herpesvirus	PCR	Detection	Yes
Infectious bursal disease (IBD)	Birnavirus	PCR	Detection	Yes
Avian leukosis	Retrovirus	PCR	Detection	Yes
Fowl cholera (pasteurellosis)	Pasteurella multocida	PCR	Detection	Yes
Reticuloendotheliosis	Retrovirus	PCR	Detection	Yes
Viral arthritis (tenosynovitis)	Reovirus	PCR	Detection	Yes
Egg drop syndrome (EDS)	Adenovirus	PCR	Detection	Yes
Avian Encephalomyelitis	Hepatovirus (picornaviridae)	PCR	Detection	Yes

Testimonials

“Correct and timely diagnosis is crucial and serves as the first building block in disease management. The assistance and benefits we receive through rapid and differential diagnosis are immeasurable.”

Dr Ambrosio T. Rubite, Company Veterinarian, Baiada Poultry

“We have used your diagnostic service for poultry diseases, especially for ILT, a disease that has become a major bird health issue in Australia. The work of your laboratory was both quick and accurate and it has been of great help to Inghams for the understanding and control of ILT … On behalf of Inghams, the largest poultry meat producer in Australia, I would like to thank you and the Australian Poultry CRC for providing this service to the Australian poultry industry.”

Dr Brian Burke, Group Veterinarian, Inghams Enterprises

“The PCR-based tests developed by the CRC give the benefit of both speed and typing,without the need for expensive and time-consuming culturing, as was often required before this service was available. This typing was invaluable in stamping out an outbreak of ILT we had recently, as it allowed quick confirmation and identification of the appropriate vaccine. It also allowed us to track where the outbreak virus was moving.”

“We also use the CRC’s PCR service to diagnose IB outbreaks. We have INB viruses that are not related to the traditional and present differently in the field. We can get a diagnosis and typing by simply posting down air dried swabs in an envelope and getting a typed result within 24 hours. Their PCR has also enabled us to investigate an IBH problem and show it is a different strain and not a vaccination failure as first thought. We are continuing to investigate this problem using PCR.”

Dr Ben Wells, Poultry Veterinarian and Acting President, Australasian Veterinary Poultry Association